Nature of interaction of dextran sulfate with lecithin dispersions and lysolecithin micelles.

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Nature of interaction of dextran sulfate with lecithin dispersions and lysolecithin micelles.

Lecithin and lecithin/cholesterol dispersions as well as lysolecithin micelles were used to provide basic information on the mechanism of the interaction of zwitterionic phospholipids with dextran sulfate. The addition of dextran sulfate to lecithin dispersions or lysolecithin micelles in the presence of Ca2+ produced insoluble complexes. At each Ca2+ concentration, the amount of insoluble comp...

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15 صفحه اول

Interaction of Fibrinogen with Dextran Sulfate

Interactions of fibrinogen with dextran sulfate, dextran, and carboxymethyl cellulose were investigated by turbidity measurement, chemical analysis, and electrophoresis. Dextran sulfate and fibrinogen combined even in the physiological pH region where both of them have negative net charges, and formed a precipitate and soluble complex. Since no complex formation was observed in the case of dext...

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Nature of the interaction of dextran sulfate with low density lipoproteins of plasma.

The nature of the interaction between dextran sulfate and low density human plasma lipoproteins of the Sr 0 to 10 class (LDL) was investigated by modifying LDL with succinic anhydride or acetic anhydride. In the absence of divalent metal ions, the formation of insoluble and soluble dextran sulfate-LDL complexes was prevented by the acylation of approximately 16% and 25%, respectively, of the LD...

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Low density lipoprotein-activated lysolecithin acylation by human plasma lecithin-cholesterol acyltransferase. Identity of lysolecithin acyltransferase and lecithin-cholesterol acyltransferase.

There is in normal plasma an enzyme activity which converts labeled lysolecithin to lecithin by an energy-independent low density lipoprotein-activated pathway. Studies were undertaken to compare the identity of this enzyme with lecithin-cholesterol acyltransferase. During purification of the enzyme by ultracentrifugation and by chromatography on high density lipoprotein affinity column, DEAE-S...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1977

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(17)40647-8